The rapid growth in content and complexity of cell imaging data creates an opportunity for synergy between experimental and computational scientists. Sharing microscopy data enable computational scientists to develop algorithms and tools for data analysis, integration and mining. These tools can be applied by experimentalists to promote discovery. We are now at the dawn of this revolution: infrastructure is constantly being developed for data standardization, deposition, sharing and analysis; journals and funding agencies mandate data deposition; data journals publish high-content microscopy datasets; quantification becomes standard in scientific publications; new analytic tools are being developed and dispatched to the community. This subgroup will bring together scientists to reflect on opening cell imaging data, and the opportunities that will come along with it.